(1. 廣東工業(yè)大學(xué) 環(huán)境科學(xué)與工程學(xué)院，廣州 510006；
2. 廣東環(huán)境保護工程職業(yè)學(xué)院，佛山 528216)
摘 要: 通過研究Cu2+濃度對嗜酸性氧化亞鐵硫桿菌(Acidithiobacillus ferrooxidans，A.f)生長特性的影響，得到在不同Cu2+濃度下A.f菌的生長曲線，探究不同Cu2+濃度對培養(yǎng)液中細(xì)菌濃度、pH、亞鐵濃度和氧化還原電位的影響。結(jié)果表明：A.f菌對Cu2+具有一定的耐受能力；當(dāng)培養(yǎng)液中ρ(Cu2+)≤0.5 g/L時，Cu2+對A.f菌的生長活性影響較小；當(dāng)ρ(Cu2+)在1.0~2.0 g/L之間變化時，A.f菌的生長活性開始受到Cu2+的影響，出現(xiàn)明顯的延遲效應(yīng)，Cu2+開始抑制細(xì)菌生長繁殖；當(dāng)ρ(Cu2+)≥3.5 g/L 時，A.f菌生長完全受到抑制，幾乎停止生長。隨著培養(yǎng)液中Cu2+濃度的增加，A.f菌生長活性受到的抑制作用也增大。依據(jù)Monod方程推導(dǎo)并建立了A.f菌在不同Cu2+濃度下的生長動力學(xué)方程，各動力學(xué)曲線的相關(guān)性較好，表明動力學(xué)方程能較好地描述不同Cu2+濃度對氧化亞鐵硫桿菌生長的影響。
關(guān)鍵字: 嗜酸性氧化亞鐵硫桿菌；銅離子；生長特性；生長動力學(xué)
（1. School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China;
2. Guangdong Polytechnic of Environmental Protection Engineering, Foshan 528216, China）
Abstract:The effect of initial copper ion concentration on the growth activity of Acidithiobacillus ferrooxidans was investigated, and the bacterial growth curves, bacteria concentration, pH, ferrous concentration and oxidation-reduction potential in the culture solution were obtained at different Cu2+ concentrations. The results show that to some extent Acidithiobacillus ferrooxidans is tolerant to copper ion. When cultured in 9K medium containing ρ(Cu2+)≤0.5 g/L, little effect on the growth and activity of Acidithiobacillus ferrooxidans is brought to copper ion. When ρ(Cu2+) is 1.0-2.0 g/L, the growth and activity of Acidithiobacillus ferrooxidans are obviously influenced by copper ion and appear delayed effects, and then copper iron begins to inhibit the bacterial growth. When the concentration of copper ion is over 3.5 g/L, the inhibiting effect on activity of Acidithiobacillus ferrooxidans is completely inhibited, bacteria almost stops growing. With the increase of Cu2+ concentration in the culture medium, the inhibiting effect on growth activity of Acidithiobacillus ferrooxidans increases. Based on the model of Monod function, the growth kinetics equation of Acidithiobacillus ferrooxidans at different copper iron concentration is built up. The correlation of kinetic curve is better, which shows that the kinetics equation can well describe the growth of Acidithiobacillus ferrooxidans at different initial copper iron concentrations.
Key words: Acidithiobacillus ferrooxidans; copper ion; growth activity; growth kinetics
