(1. 中南大學 湘雅三醫(yī)院，長沙 410013；
2. 中南大學 粉末冶金國家重點實驗室，長沙 410083；
3. 中南大學 醫(yī)用材料與器械研究中心，長沙 410013)
摘 要: 以硝酸鈣、磷酸氫二氨等為反應原料，采用水熱合成法制備羥基磷灰石(HAP)納米顆粒，對該HAP顆粒以及添加精氨酸(Arg)或摻雜少量稀土離子鋱(Tb)/銪(Eu)對HAP顆粒的形貌修飾等進行研究，采用透視電鏡(TEM)、X射線衍射儀(XRD)、傅立葉紅外光譜儀(FTIR)等，對制備樣品的結晶性、粒度、分散性等進行分析測試。結果表明:精氨酸表面修飾改變HAP納米顆粒的表面Zeta電位，從而在一定程度上抑制HAP的生長速率；少量稀土離子Eu/Tb摻雜并不影響HAP/Arg納米顆粒產物的結構，均為單一的HAP物相，所合成產物的粒徑也為納米級。經稀土金屬鋱/銪摻雜的精氨酸表面修飾的HAP納米顆粒可作為基因轉染載體。
關鍵字: 羥基磷灰石；水熱合成；精氨酸修飾；稀土摻雜
（1. The Third Xiangya Hospital, Central South University, Changsha 410013, China;
2. State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China;
3. Research Center for Medical Material and Instruments, Central South University, Changsha 410013, China）
Abstract:Nano-hydroxyapatite(HAP) was prepared by a hydrothermal method with calcium nitrate, diammonium hydrogen phosphate as raw material, and to study its characteristics for morphology modification of arginine-functionalized and doped with rare earth, such as Eu3+ or Tb3+. The crystallization, grain size and dispersibility of the sample HAP were analyzed and discussed. The results show that the surface Zeta potential of arginine-functionalized HAP is changed, and the growth rate of HAP is inhibited to a certain extent during the synthesis. The structure of HAP/Arg is not affected during the synthesis doped by a small amount of rare earth ions, such as Eu3+ or Tb3+. All these samples have single phase of HAP with good dispersibility. The synthesized HAP is also nano-sized level. Nano-hydroxyapatite with arginine functionalized and rare earth doped, such as Eu3+ or Tb3+, is suitable for the application of gene delivery as a gene carrier.
Key words: hydroxyapatite; hydrothermal synthesis; arginine modification; rare-earth doping
