(1. 中南大學 資源加工與生物工程學院，長沙 410083;
2. 中南大學 生物冶金教育部重點實驗室，長沙 410083)
摘 要: 采用3種中等嗜熱微生物:喜溫硫桿菌(Acidithiobacillus caldus，A.c)、嗜鐵鉤端螺旋菌(Leptospirillum ferriphilu，L.f)、嗜熱氧化硫化桿菌(Sulfobacillus thermosulfidooxidans，S.t)對黃銅礦精礦進行浸出。探討浸出過程中的微生物生長優(yōu)化及攪拌反應(yīng)器浸出條件優(yōu)化。微生物最佳生長條件如下:生長溫度為45 ℃、初始pH為1.5。馴化過的浸礦細菌的生長及浸出率明顯高于未馴化的，馴化后浸出率在礦漿濃度為50 g/L時達到最大，為94.00%;當?shù)V漿濃度達到100 g/L時，銅的浸出率穩(wěn)定在80%左右。攪拌反應(yīng)器的最優(yōu)化浸出條件如下:攪拌速度350 r/min，充氣強度500 mL/min。在此條件下，對黃銅礦精礦進行浸出，浸出時間為30 d時，最終銅離子濃度為17.36 g/L，銅的浸出率為85.60%。
關(guān)鍵字: 黃銅礦;中等嗜熱菌;生物浸出
（1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;
2. Key Laboratory of Biometallurgy, Ministry of Education, Central South University, Changsha 410083, China）
Abstract:The bioleaching of chalcopyrite concentrate in the presence of three different moderate thermophilic bacteria such as Acidithiobacillus caldus (A.c), Leptospirillum ferriphilu (L.f) and Sulfobacillus thermosulfidooxidans (S.t), including the optimal conditions of microbial growth and the optimization of stirred reactor during the bioleaching process, was investigated. The results show that the best growth conditions of bacteria are as follows, temperature of 45 ℃ and initial pH of 1.5. The microbial growth of the domesticated bacteria and the bioleaching rate using them are significantly higher than those of the non-domesticated bacteria. The leaching rate using the domesticated strains reaches the maximum 94.00% with the pulp density of 50 g/L. The leaching rate of copper stabilizes at about 80% when the pulp density reaches 100 g/L. The optimal parameters of stirred reactors are as follows:stirring speed of 350 r/min and aeration intensity of 500 mL/min. The final concentration of copper ions is 17.36 g/L and the leaching rate of copper is 85.60% for the chalcopyrite bioleaching for 30 d under these conditions.
Key words: chalcopyrite; moderate thermophilic bacteria; bioleaching
