(1. 中南大學(xué) 資源加工與生物工程學(xué)院，長沙410083;
2. 中南大學(xué) 生物冶金教育部重點(diǎn)實(shí)驗(yàn)室，長沙410083)
摘 要: 采用微生物浸出技術(shù)，選用4種中度嗜熱浸礦菌:嗜鐵鉤端螺旋菌(Leptospirillum ferriphilum)，嗜酸喜溫硫桿菌(Acidithiobacillus caldus)，嗜熱硫氧化硫化桿菌(Sulfobacillus thermosulfidooxidans)和嗜熱嗜酸鐵質(zhì)菌(Ferroplasma thermophilum)，作為混合菌回收廢棄線路板分選尾渣中的金屬銅。通過搖瓶浸出實(shí)驗(yàn)，研究混合菌在不同濃度分選尾渣中的浸出過程，并探究初始pH、初始Fe2+質(zhì)量濃度、培養(yǎng)溫度及粉末粒徑對(duì)銅浸出的影響;并將優(yōu)化條件應(yīng)用至3L攪拌槽中，實(shí)現(xiàn)浸出體系的擴(kuò)大。結(jié)果表明:搖瓶馴化過程中，混合菌種在分選尾渣中的生長情況較好，且能實(shí)現(xiàn)金屬銅的有效浸出;在優(yōu)化參數(shù)為初始pH 1.5、ρ(Fe2+) 1 g/L、45 ℃條件下的放大實(shí)驗(yàn)中，浸出至第7 d時(shí)，銅浸出率最高達(dá)到93.09%。
關(guān)鍵字: 微生物浸出;混合菌種;廢棄線路板分選尾渣;銅浸出率
（1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;
2. Key Laboratory of Biometallurgy, Ministry of Education, Central South University, Changsha 410083, China）
Abstract:According to micro-bioleaching technology, four moderately thermoacidphilic strains, i.e., Leptospirillum ferriphilum, Acidithiobacillus caldus, Sulfobacillus thermosulfidooxidans and Ferroplasma thermophilum, were used as mixed culture to recover copper from nonmetal slags, which was separated from waste printed circuit boards(WPCBs) by water power shaker. Firstly, the bioleaching experiments with different concentration of nonmetal slags in shake flasks were carried out. Then, the effects of initial pH, initial Fe2+ concentration, incubation temperature and particle size on the bioleaching were investigated. Finally, under the optimized conditions, the bioleaching experiment was conducted in a stirred tank with volume of 3L to achieve system amplified. The results show that the growth of mixed culture is good in the existence of nonmetal slags. Besides, under the conditions of initial pH 1.5, initial ρ(Fe2+) 1 g/L and 45 ℃, 93.09% copper recovery is achieved in 7 d in the amplified system.
Key words: micro-bioleaching; mixed culture; waste printed circuit boards separated slags; copper recovery
