(1. 中南大學(xué)資源加工與生物工程學(xué)院，長(zhǎng)沙410083；
2. 中南大學(xué)教育部生物冶金重點(diǎn)實(shí)驗(yàn)室，長(zhǎng)沙410083)
摘 要: 與傳統(tǒng)微生物生態(tài)學(xué)分析方法相比，近幾年高通量測(cè)序以速度快、通量高、低成本等特征在微生物多樣性檢測(cè)方面得到了充分應(yīng)用，并采用此方法研究溫度對(duì)微生物浸出贊比亞一種低品位銅礦的效率和群落生態(tài)的影響。結(jié)果表明：在30℃和45℃浸出條件下，銅浸出率分別為64.2%和69.4%，45℃條件下獲得了較高銅浸出率。采用高通量Illumina Miseq測(cè)序技術(shù)分析30℃和45℃浸出條件下微生物群落結(jié)構(gòu)及其動(dòng)態(tài)變化，當(dāng)浸出溫度為30℃，Acidithiobacillus caldus和Acidiphilium sp.是主要浸礦菌；當(dāng)浸出溫度為45℃時(shí)，Sulfobacillus sp.和Acidithiobacillus caldus是優(yōu)勢(shì)種群。
關(guān)鍵字: 銅礦；溫度；生物浸出；群落動(dòng)態(tài)；Illumina Miseq測(cè)序
（1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;
2. Key Laboratory of Biometallurgy, Ministry of Education, Central South University, Changsha 410083, China）
Abstract:The main features of high-throughput sequencing are high speed, high-throughput and low cost compared with the traditional microbial ecology analysis method. In recent years, the high-throughput sequencing technology gets full uses in the investigation of microbial diversity. The effects of temperature on bioleaching of low grade copper ore and the dynamics of microbial community from Zambia were studied. The results show that, at30℃ and 45℃, the copper leachingratesare 64.2% and 69.4%, respectively. The rise of temperature results in higher copper leaching rate at 45℃. The shift of microbial community at 30℃ and 45℃ during bioleaching process was analyzed by Illumina Miseq sequencing platform. The results show that Acidithiobacillus caldus and Acidiphilium sp. are the main microorganisms at 30℃. While at 45℃, Sulfobacillus sp. and Acidithiobacillus caldus dominate in the microbial community.
Key words: copper ore; temperature; bioleaching; microbial community dynamics; Illumina Miseq sequencing
