(中南大學(xué) 資源加工與生物工程學(xué)院，長沙 410083)
摘 要: 為研究中國20株嗜酸氧化亞鐵硫桿菌菌株的基因多樣性，以及基因指紋技術(shù)分類與嗜酸氧化亞鐵硫桿菌生理生化特性的相關(guān)性，使用20種隨機(jī)引物，通過PCR擴(kuò)增20株菌株的基因組DNA，并分析凝膠電泳條帶的有無，使用Jaccard系數(shù)和UPGMA分析菌株間相關(guān)性。20種引物產(chǎn)生的條帶將20株菌株分成了5組，菌株間相關(guān)性系數(shù)最小為35.1%(YTW與DXS)，而相似系數(shù)最大為96.9%(BY-0502與DXS，DY26與DY30)。結(jié)果表明：20株來自不同地區(qū)的嗜酸氧化亞鐵硫桿菌株具有較高的多樣性，并且親緣性分類與各菌株取樣地以及最大銅離子耐受能力分類，有較高的分類相似性，說明RAPD技術(shù)擁有在嗜酸氧化亞鐵硫桿菌內(nèi)分類鑒定菌株的能力。
關(guān)鍵字: 嗜酸氧化亞鐵硫桿菌；RAPD；多樣性
（School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China）
Abstract:To study the genome diversity of 20 Acidithiobacillus ferrooxidans strains in China, and the relationship between the genome classification and physiological-biochemical characteristic of Acidithiobacillus ferrooxidans, DNA was amplified by PCR and twenty random primers, amplification products were separated by gel electrophoresis and photographed under UV light. The RAPD (Randomly Amplified Polymorphic DNA) profiles were compared on the basis of the presence or absence of each DNA band, and strain diversity was calculated using the Jaccard’s coefficient and UPGMA (Unweighted Pair-Group Arithmetic Average Clustering) cluster analysis. The primers used in this study grouped the 20 strains into five major groups. Similarities between the strains ranged from 35.1%(YTW and DXS) to 96.9%(BY-0502 and DXS，DY26 and DY30). These results indicate that the 20 strains have a high degree of genomic diversity and the RAPD markers related to characteristics, such as metal resistance and geographical locations. The RAPD procedure is a powerful technique to assess strain variability in Acidithiobacillus ferrooxidans.
Key words: Acidithiobacillus ferrooxidans; RAPD; diversity
