(廣東工業(yè)大學(xué) 環(huán)境科學(xué)與工程學(xué)院，廣州 510006)
摘 要: 從廣東凡口鉛鋅礦尾礦庫周邊土壤中分離出一株能高效降解松醇油的細(xì)菌KS-1，并進(jìn)行人工配制含松醇油的模擬廢水的化學(xué)需氧量(COD)降低條件優(yōu)化試驗(yàn)研究。形態(tài)學(xué)和生理學(xué)分析表明：該KS-1菌株為革蘭氏陽性好氧菌，呈細(xì)胞桿狀、芽孢橢圓或柱狀；接觸酶、硝酸鹽還原和V-P測定呈陽性反應(yīng)；能利用葡萄糖、木糖、甘露醇、檸檬酸鹽、淀粉和明膠生長，不能利用吲哚、卵黃卵磷脂酶、苯丙氨酸脫氨酶和丙酸鹽生長。16SrDNA序列分析表明：該菌株與Bacillus subtilis AF0907的相似性達(dá)到100%，最終鑒定菌株KS-1為枯草芽孢桿菌(Bacillus subtilis AF0907)，登錄號(hào)為GU272021。正交試驗(yàn)優(yōu)化結(jié)果表明：菌株KS-1降低松醇油廢水COD工藝最佳條件為pH4，接種量 15%，搖床轉(zhuǎn)速200 r/min，松醇油濃度300 mg/L，在此條件下反應(yīng)4 d，COD去除率達(dá)到65.91%。
關(guān)鍵字: 松醇油；選礦廢水；菌種鑒定；枯草芽孢桿菌
HUANG Shao-song, YIN Guang-cai, TIAN Jing, ZENG Jia-jun, XU Ping-ting
（School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China）
Abstract:A strain KS-1 capable of highly degrading pine oil was isolated from the soil collected from tailings reservoir in Fankou Lead-zinc Mine of Guangdong province, and the best degradation conditions of pine oil wastewater (chemical oxygen demand, COD) were investigated. The analysis of morphology and physiology shows that the strain KS-1 is gram-positive and aerobic bacterium and rhabditiform, which can grow by using glucose, xylose, mannitol, citrate, starch and gelatin and cannot grow with indole, yolk lecithinase, phenylalanine deaminase and propionate. Based on analysis of phenotype, physiological and biochemical characters and 16SrDNA, KS-1 is identified as Bacillus subtilis AF0907 due to its 16SrDNA sequence, GU272021, which has the similarity of 100% to Bacillus subtilis AF0907. According to the orthogonal optimization test, the degradation conditions are as follows: pH 4, inoculating ratio 15%, rotation speed 200 r/min, pine oil concentration 300 mg/L, with COD removal rate up to 65.91% in 4 d.
Key words: pine oil; flotation wastewater; strain identification; Bacillus subtilis
