(1. 湖南有色金屬研究院，長沙 410015；2. 中南大學 冶金科學與工程學院，長沙 410083)
摘 要: 通過對培養(yǎng)基的優(yōu)化，提出并研究直接添加培養(yǎng)基激活土著微生物的活性來進行鉻渣堆場污染土壤的原位微生物修復新方法，并探討土壤環(huán)境對土著微生物修復Cr(Ⅵ)效果的影響。研究表明：在每千克土壤中碳源葡萄糖投加量為5 g、氮源化合物A投加量為5 g、溫度為30 ℃、土與液質量比為1:1的情況下，土著微生物可被迅速激活，在第4天時能基本去除土壤的中水溶性Cr(Ⅵ)；培養(yǎng)基初始pH值和不添加無機鹽氯化鈉不影響其修復效果。
關鍵字: 鉻渣堆場；鉻污染；土著微生物；原位修復
（1. Hunan Research Institute of Nonferrous Metals, Changsha 410015, China;
2. School of Metallurgical Science and Engineering, Central South University, Changsha 410083, China）
Abstract:Based on the optimization of culture medium composition, the growth conditions and ability of Cr(Ⅵ) reduction, the in-situ remediation of Cr-contaminated soil can be achieved by adding culture medium in soils to stimulate the activity of indigenous microorganism. The results show that the optimal conditions for the Cr(Ⅵ) reduction by indigenous microorganism are 5 g glucose and 5 g nitrogenous compounds A per kilogram soil at 30 ℃ and the mass ratio of soil to water is 1:1. Under the optimal condition, the water soluble Cr(Ⅵ) in the soil contaminated by chromium-containing slag heap is completely removed in the fourth day. The initial pH value of culture medium do not affect Cr(Ⅵ) reduction. The inorganic salt does not need to be added into the culture medium when the in-situ remediation of Cr(Ⅵ) is carried out by the indigenous microorganism.
Key words: chromium-containing slag heap; Cr-contamination; indigenous microorganism; in-situ remediation
