(1. 中南大學(xué) 湘雅三醫(yī)院，長沙 410013；
2. 中南大學(xué) 粉末冶金國家重點實驗室，長沙 410083)
摘 要: 采用堿熱處理和仿生沉積HA對注射成形多孔鈦進行表面處理，研究改性后多孔鈦的細胞毒性及其成骨細胞黏附、增殖和分化的影響。結(jié)果表明：改性后多孔鈦無細胞毒性，不同孔隙度多孔鈦的細胞黏附形貌不同，復(fù)合培養(yǎng)7 d后，5%孔隙度組細胞平鋪于材料表面，30%孔隙度組細胞多呈長梭形，60%孔隙度組MG63細胞偽足以錨狀牢固附著于孔壁的顆粒之上，形成細胞橋；成骨細胞在高孔隙度組表面細胞的增殖數(shù)高于低孔隙度組表面細胞的；復(fù)合培養(yǎng)7 d與14 d，5%孔隙度組與30%孔隙度組的上清液中堿性磷酸酶(Alkaline phosphatase, ALP)含量無明顯差別(p＜0.05)，而60%孔隙度組的ALP含量高于低孔隙度組的。
關(guān)鍵字: 生物材料；醫(yī)用鈦合金；多孔鈦；孔隙度；黏附；增殖；堿性磷酸酶
（1. Third Xiangya Hospital, Central South University, Changsha 410013, China;
2. State Key Laboratory of Powder Metallurgy, Central South University, Changsha 410083, China）
Abstract:The metal injection molding (MIM) method was used to fabricate porous Ti implants, the surface modification of the porous titanium was performed using alkali-heat treatment and biomimetic hydroxyapatite (HA) deposition. The influence of modified porous titanium on the cell toxicity, adhesion, proliferation and differentiation of the osteoblast cells were investigated. The results show that the modified porous titanium is nontoxic to cells. The morphologies of the cells vary with change of porosity of porous titanium. After cultured 7 d, MG63 is flat on the surface of 5% porosity titanium implant and extends to amphi-edge of the pore on the surface of 30% porosity titanium implant. The titanium implant with porosity of 60% can be clearly noticed that the pseudopodias adhere deeply to the hole wall and form the cell bridges, and the cells can grow into the pores. The number of the cell proliferation in high porosity groups is higher than that in low group. The alkaline phosphatase(ALP)content is influenced by the porosity of porous titanium, there is no significant difference between 5% and 30% porosity group (p＜0.05), the ALP content of 60% porosity group is higher than that of low porosity group.
Key words: biomaterial; medical titanium alloy; porous titanium; porosity; adhesion; proliferation; alkaline phosphatase
