(1. 中南大學 資源加工與生物工程學院，長沙 410083；
2. 中南大學 生物冶金教育部重點實驗室，長沙 410083)
摘 要: 研究3株極端嗜熱古菌(金屬硫葉菌，Sulfolobus metallicus JCM 9184；瑟杜生金屬球菌，Metallosphaera sedula JCM 9185和萬座酸菌，Acidianus manzaensis YN25)在不同起始pH值和不同溫度條件下對黃銅礦的混合浸出，并對浸礦過程中混合菌群落的動態(tài)演替進行分析。結果表明：在起始pH 1.5時的銅浸出率明顯高于在起始pH 2.5時的銅浸出率，而65 ℃條件下的銅浸出率高于75 ℃時的銅浸出率。利用限制性長度多態(tài)性(RFLP)分析65 ℃、起始pH 1.5條件下的微生物群落演替，結果顯示：在黃銅礦的浸出前期Sulfolobus metallicus是占據優(yōu)勢的菌種，而到后期Acidianus manzaensis的比例則會上升，并最后取代Sulfolobus metallicus成為優(yōu)勢種。
關鍵字: 生物浸出；黃銅礦精礦；極端嗜熱微生物；微生物群落；演替
mixed thermophilic culture and succession of
microbial community during leaching process
（1. School of Minerals Processing and Bioengineering, Changsha 410083, China;
2. Key Laboratory of Biometallurgy, Ministry of Education, Central South University, Changsha 410083, China）
Abstract:A mixed thermophilic culture consisting of Sulfolobus metallicus JCM 9184, Metallosphaera sedula JCM 9185 and Acidianus manzaensis YN25 was used to study the combined effect of pH value and temperature in bioleaching of a chalcopyrite concentrate. The results show that the copper extraction at initial pH 1.5 is higher than that at intial pH 2.5. The copper extraction at 75 ℃ is lower than that at 65 ℃, but the lower copper extraction at 75 ℃ is not due to the inhibition of the high temperature. The succession of the microbial community during the leaching process was analysed by restriction fragment length polymorphism (RFLP). The analysis shows that Sulfolobus metallicus is dominant in the microbial community at the early stage of leaching, whereas Acidianus manzaensis thrives at the latter stage. This phenomenon might be caused by the change of ferrous ion concentration during leaching process.
Key words: bioleaching; chalcopyrite concentrate; extremely thermophilic microorganisms; microbial community; succession
