(中南大學(xué) 資源加工與生物工程學(xué)院 生物冶金教育部重點(diǎn)實(shí)驗(yàn)室，長(zhǎng)沙 410083)
摘 要: 以嗜酸氧化亞鐵硫桿菌(Acidithiobacillus ferrooxidans, ATCC23270)為對(duì)象，研究NO3−、SO42−、Cl−和PO43−對(duì)Acidithiobacillus ferrooxidans生長(zhǎng)和硫氧化活性的影響。結(jié)果表明：不同陰離子對(duì)Acidithiobacillus ferrooxidans硫氧化表觀活性的影響效應(yīng)不同，其影響由大到小的順序依次為Cl−，NO3−，SO42−和PO43−；在高濃度PO43−的脅迫(300 mmol/L)下，細(xì)菌所表達(dá)蛋白質(zhì)的總數(shù)量減少，但同時(shí)表達(dá)一些特異性蛋白質(zhì)，這說(shuō)明細(xì)菌可通過(guò)抑制一些在正常條件下表達(dá)的蛋白質(zhì)，同時(shí)表達(dá)一些新的蛋白質(zhì)來(lái)應(yīng)對(duì)無(wú)機(jī)離子的脅迫效應(yīng)。
關(guān)鍵字: 嗜酸氧化亞鐵硫桿菌；陰離子；脅迫效應(yīng)
Acidithiobacillus ferrooxidans
（Key Laboratory of Biometallurgy, Ministry of Education, School of Minerals Processing and Bioengineering,
Central South University, Changsha 410083, China）
Abstract:The effects of NO3−, SO42−, Cl− and PO43− on the growth of Acidithiobacillus ferrooxidans and sulfur oxidation activity were investigated. The results show that the anions have different effects on the sulfur oxidation activity, and the inhibition effect sequence from great to little is Cl−, NO3−, SO42−, PO43−. The total proteins of Acidithiobacillus ferrooxidans grown under PO43− threatening condition (300 mmol/L) are down-regulated, but specific proteins are observed, when compared with normal condition. These specific proteins may take part in the response to the stress effect of anions.
Key words: Acidithiobacillus ferrooxidans; anion; stress effect
