(1. 中南大學(xué) 冶金科學(xué)與工程學(xué)院，長沙 410083；
2. 中南大學(xué) 水污染控制技術(shù)湖南省重點(diǎn)實(shí)驗(yàn)室，長沙 410004)
摘 要: 在前期從鉻渣污染土壤中分離篩選出高效還原Cr(Ⅵ)的土著微生物(Pannonibacter phragmitetus)的基 礎(chǔ)上，通過單因素實(shí)驗(yàn)對所篩選的土著微生物修復(fù)模擬鉻污染土壤效應(yīng)及其影響因素進(jìn)行研究。結(jié)果表明： 土著微生物對土壤中Cr(Ⅵ)的修復(fù)受pH值、微生物接種量和初始Cr(Ⅵ)濃度的影響；pH值越高、微生物接種量越大而初始Cr(Ⅵ)濃度越低時，Cr(Ⅵ)的修復(fù)速率越快；所篩選的土著微生物在316 h內(nèi)就能夠完全還原污染土壤中濃度為360 mg/kg的Cr(Ⅵ)；Cr(Ⅵ)的修復(fù)是土著微生物(Pannonibacter phragmitetus)通過酶促反應(yīng)對Cr(Ⅵ)的直接還原所致。
關(guān)鍵字: 鉻(Ⅵ)污染土壤；鉻(Ⅵ)污染修復(fù)；Pannonibacter phragmitetus
contaminated soils
（1. School of Metallurgical Science and Engineering, Central South University, Changsha 410083, China;
2. Hunan Key Laboratory of Water Pollution Control Technology, Central South University, Changsha 410004, China）
Abstract:Based on the isolation and screening of chromium-reduction bacterium (Pannonibacter phragmitetus) in the previous research, this strain was used to remediate Cr(Ⅵ) in the contaminated soils. The Cr(Ⅵ) reduction effectiveness by Pannonibacter phragmitetus and its factors were investigated using single factor experiment. The results show that the Cr(Ⅵ) remediation is affected by pH value, inoculation amount of Pannonibacter phragmitetus and initial Cr(Ⅵ) concentration. The remediation rate increases with increasing pH value and inoculation amount of mircrobes, and increases with decreasing initial Cr(Ⅵ) concentration. Pannonibacter phragmitetus can completely reduce 360 mg/kg of Cr(Ⅵ) in the contaminated soil within 316 h. Cr(Ⅵ) remediation in soils is attributed to the direct reduction of Cr(Ⅵ) via enzymatic reaction of Pannonibacter phragmitetus.
Key words: Cr(Ⅵ)-contaminated soil; Cr(Ⅵ) remediation; Pannonibacter phragmitet
