(1. 中國科學(xué)院 過程工程研究所 生化工程國家重點實驗室，北京 100080；
2. 北京科技大學(xué) 土木與環(huán)境工程學(xué)院，北京 100083)
摘 要: 以微生物燃料電池為研究工具，研究Rhodoferax ferrireducens(R.f )異化還原釩(Ⅴ)的過程。在對R.f進(jìn)行NaVO3耐性馴化實驗的基礎(chǔ)上，考查NaVO3廢水濃度、pH值和溫度對R.f還原釩(Ⅴ)的影響。結(jié)果表明：適量釩元素對微生物生長具有促進(jìn)作用，微生物的生長趨勢及周期與釩(Ⅴ)的還原趨勢及周期基本相同；初期由于釩化合物的生物效應(yīng)促進(jìn)葡萄糖消耗量增加，葡萄糖氧化分解過程產(chǎn)生小分子有機(jī)酸，使溶液pH降低，但釩(Ⅴ)還原過程需要H+的參與，且R.f異化作用也消耗部分小分子有機(jī)酸，后期溶液pH升高。經(jīng)優(yōu)化其培養(yǎng)條件為：溫度30 ℃、攪拌速度150 r/min、pH為7.5時，在NaVO3含量為300 mg/L的釩廢水中，R.f對釩(Ⅴ)的還原率為76%，細(xì)菌總數(shù)達(dá)到1.1×109 cfu/mL。
關(guān)鍵字: 釩冶金廢水；Rhodoferax ferrireducens；微生物燃料電池；異化還原
（1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering,
Chinese Academy of Sciences, Beijing 100080, China;
2. School of Civil and Environmental Engineering, University of Science and Technology Beijing,
Beijing 100083, China）
Abstract:The process of Rhodoferax ferrireducens (R.f ) dissimilatory reduction of V5+ in waste water was studied by the means of microbial fuel cell (MFC). Based on the inoculation test of tolerance to NaVO3 of R.f, the effects of NaVO3 concentration in the wastewater, pH and temperature on R.f reducing V5+ were investigated. The results show that adequate vanadium can promote the growth of microbes, and the tendency and cycle of R.f growth are essentially the same as that of V5+ reducing. In beginning period, the bioeffect of vanadium compound increases the consumption of glucose, and the glucose is oxidized to the small molecule organic acids, which leads to pH value fall. But H+ should be engaged in V5+ reduction, and the dissimilatory reduction of R.f will also consume some small molecule organic acids, which makes pH increase later. The optimized culture conditions are as follows: temperature 30 ℃, stirring speed 150 r/min, pH 7.5, NaVO3 concentration in the vanadium wastewater 300 mg/L. The reduction rate of V5+ by R.f is 76%, and the total R.f count reaches 1.1×109 cfu/mL.
Key words: vanadium metallurgical waste water; Rhodoferax ferrireducens; microbial fuel cell; dissimilatory reduction
