黃菊芳1, 2, 胡岳華1, 2
(1. 中南大學(xué) 資源加工與生物工程學(xué)院, 長(zhǎng)沙 410083;
2. 中南大學(xué) 生物冶金教育部重點(diǎn)實(shí)驗(yàn)室, 長(zhǎng)沙 410083)
摘 要: 從廣西銅坑礦區(qū)多金屬復(fù)雜硫化礦礦坑水中分離得到了一株嗜酸氧化亞鐵硫桿菌(暫命名為TK), 該菌株為革蘭氏陰性細(xì)菌, 短桿狀, 菌體大小為（0.4±0.1） μm×（1.4±0.2） μm, 最適生長(zhǎng)溫度為25~30 ℃, 最適pH值為2.0, 化能自養(yǎng)型, 能利用亞鐵、 單質(zhì)硫和葡萄糖生長(zhǎng), 不能利用硫代硫酸鈉、 蛋白胨生長(zhǎng)。測(cè)定其亞鐵和元素硫的氧化能力, 并考察它對(duì)鐵閃鋅礦的浸出效果。 以16SrDNA序列同源性為基礎(chǔ)構(gòu)建了包括15株已報(bào)道菌種和其他菌屬菌株作為外來群在內(nèi)的系統(tǒng)發(fā)育樹。 結(jié)果表明： TK菌株處于單獨(dú)的一個(gè)分支內(nèi), 與嗜酸氧化亞鐵硫桿菌株D2最為相似, 相似性為98.67%, 推測(cè)TK菌株為嗜酸氧化亞鐵硫桿菌的一個(gè)新菌系。該菌系的分離為嗜酸氧化亞鐵硫桿菌多樣性和生態(tài)分布研究提供了一種新材料。
關(guān)鍵字: 嗜酸氧化亞鐵硫桿菌； 生物浸出； 系統(tǒng)發(fā)育樹； 16SrDNA
Acidithiobacillus ferrooxidans strain from complex sulfide mines
HUANG Ju-fang1, 2, HU Yue-hua1, 2
（1. School of Minerals Processing and Bioengineering,
Central South University, Changsha 410083, China;
2. Key Laboratory of Biometallurgy of Ministry of Education,
Central South University, Changsha 410083, China）
Abstract: An Acidithiobacillus ferrooxidans strain TK was isolated from the Tong Ken area, Guangxi province in China. The cell of TK strain is Gram negative and rod-shaped in (0.4±0.1） μm×(1.4±0.2） μm. The optimum growth temperature is 25-30 ℃, and the optimum growth pH is 2.0. The TK strain can grow autographically by using Fe2+, sulfur, glucose as sole energy sources, however, can not grow with NaS2O3 and peptone. The oxidation of Fe2+, sulfur and bioleaching marmatite with this strain was studied. A phylogenetic tree was constructed by comparing with the published 16SrDNA sequences of the relative bacteria species. In the phylogenetic tree, the TK strain is in a new branch which has the closest relative to the Acidithiobacillus ferrooxidans strain D2 with 98.67% sequence similarity. These results reveal that the TK strain may be a new strain of Acidithiobacillus ferrooxidans. It offers a new material in order to study the polymorphism and bionomics of bioleahing microbes.
Key words: Acidithiobacillus ferrooxidans(A.f); bioleaching; phylogenetic tree; 16SrDNA
