賈春云1, 蘇秀娟1, 秦玉春1
(1. 東北大學 資源與土木工程學院, 沈陽 110004;
2. 鞍山科技大學 土木與交通工程學院, 鞍山 114044)
摘 要: 研究了諾卡氏菌在不同影響因素條件下, 在黃鐵礦和閃鋅礦表面的選擇性吸附。結(jié)果表明： 培養(yǎng)120 h后諾卡氏菌達到最大生長量； 諾卡氏菌在黃鐵礦和閃鋅礦表面發(fā)生明顯的選擇性吸附, 在黃鐵礦表面的最大吸附率可達到96.99％, 而在閃鋅礦表面的吸附率大都在20％以下； 諾卡氏菌在兩種礦物表面于20 min內(nèi)即可達到吸附平衡； pH值是影響諾卡氏菌在兩種礦物表面發(fā)生選擇性吸附的關鍵因素, pH在4～10之間時, 選擇性吸附現(xiàn)象明顯； 礦漿濃度超過6 g/L時有利于發(fā)生選擇性吸附； 細胞懸濁液濃度、攪拌速度和溫度對吸附效果影響不大, 均可產(chǎn)生選擇性吸附。 掃描電鏡檢測結(jié)果表明, 諾卡氏菌細胞表面的菌絲是重要的吸附部位； 紅外光譜分析結(jié)果表明, 諾卡氏菌表面含有—OH、 —NH2、 C==O、 P==O、 S==O等活性官能團, 它們在吸附過程中起重要作用。
關鍵字: 黃鐵礦； 閃鋅礦； 諾卡氏菌； 選擇性吸附； 生物浮選
surface of pyrite and sphalerite
JIA Chun-yun1, SU Xiu-juan1, QIN Yu-chun1
（1. School of Resources and Civil Engineering,
Northeastern University, Shenyang 110004, China;
2. School of Civil and Traffic Engineering,
Anshan University of Science and Technology, Anshan 114044, China）
Abstract: The selective adsorption of Nocardia on the surface of pyrite and sphalerite was studied under various conditions. The results show that the maximum growth quantity of Nocardia can be achieved after culturing 120 h and obvious selective adsorption of Nocardia on the surface of pyrite and sphalerite can be detected. The maximum adsorption rate on the surface of pyrite is 96.99%, whereas it is less than 20% in the case of sphalerite. Adsorption equilibrium can be attained within 20 min on the surfaces of the two minerals. pH value is the key factor influencing upon selective adsorption, and the difference of adsorption quantity on the surfaces of two minerals is apparent when pH is between 4 and 10. Slurry concentration over 6 g/L is favorable to selective adsorption. Nocardia cell concentration, stirring speed and temperature have no obviously effect on selective adsorption. SEM result indicates that the mycelium on the surface of ectoblast of Nocardia cell is the important attachable site. IR spectrum shows that the functional groups such as —OH, —NH2, C==O, P==O, S==O, etc make vital contribution on adsorption process.
Key words: pyrite； sphalerite； Nocardia； selective adsorption； bioflotation
