(1.中南大學(xué) 資源加工與生物工程學(xué)院，長(zhǎng)沙 410083；2.中南大學(xué) 生物冶金教育部重點(diǎn)實(shí)驗(yàn)室，長(zhǎng)沙 410083)
摘 要: 從云南騰沖酸性熱泉分離出一株嗜酸熱異養(yǎng)菌TC-2。該菌呈長(zhǎng)桿狀，細(xì)胞大小為(2.0~5.0) μm×(0.3~0.5) μm，革蘭氏染色反應(yīng)為陰性，能形成芽孢。該菌生長(zhǎng)溫度范圍為45~70 ℃，最適生長(zhǎng)溫度為55 ℃，適宜生長(zhǎng)的pH值為1.5~4.0，最適宜生長(zhǎng)的pH值為2.5，該菌能利用多種有機(jī)物生長(zhǎng)。16S rDNA序列分析顯示其與Alicyclobacillus sendaiensis的16S rDNA相似性大于99%。該菌自身沒有浸礦作用，但對(duì)Acidianus manzaensis、Sulfolobus metallicus和Metallosphaera sedula等高溫浸礦菌的浸礦有一定促進(jìn)作用。
關(guān)鍵字: 嗜酸熱異養(yǎng)菌(Alicyclobacillus sendaiensis)；分離；鑒定；黃銅礦；生物浸出
（1. School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China;2. Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China）
Abstract:A heterotrophic acidothermophilic bacterium TC-2 was isolated from an acid spring sample collected in Tengchong, Yunnan, China. The gram-negative-staining cells of the strain were straight long rods, 2.0−5.0 µm in length and 0.3−0.5 µm in diameter. The temperature range for growth was from 45 ℃ to 70 ℃ with an optimum temperature of 55 ℃. The pH range for growth at 55 ℃ was from 1.5 to 4.0 with an optimum at 2.5. This bacterium can use a wide variety of organic compounds to support fast growth, but can not utilize chalcopyrite as energy source. 16s rRNA gene Analysis reveal that this strain closely relates to Alicyclobacillus sendaiensis, with over 99% sequence similarity between each other. Co-bioleaching of chalcopyrite with Acidianus manzaensis，Sulfolobus metallicus and Metallosphaera sedula shows that the strain TC-2 can improve leaching rates of the sulphide minerals.
Key words: heterotrophic acidothermophile(Alicyclobacillus sendaiensis); isolation; characterization; bioleaching; chalcopyrite
