(中南大學(xué) 資源加工與生物工程學(xué)院，長沙 410083)
摘 要: 采用連續(xù)稀釋法分別從江西德興銅礦、湖北大冶銅礦和廣西大廠這3處酸性礦坑水樣品中分離得到三株嗜酸性短桿狀細菌，其形態(tài)及生理特征與嗜酸性氧化亞鐵硫桿菌相似，16S rRNA系統(tǒng)學(xué)分析進一步證實這種細菌屬于硫桿菌屬的嗜酸性氧化亞鐵硫桿菌。研究表明：陰離子對嗜酸氧化亞鐵硫桿菌的Fe2+氧化能力具有一定影響，其影響順序由大到小依次為：NO3＞Cl−＞SO4；不同菌株對陰離子的耐受能力存在明顯的差異。
關(guān)鍵字: 嗜酸氧化亞鐵硫桿菌； ；Cl−；
（School of Resources Processing and Bioengineering, Central South University, Changsha 410083, China）
Abstract:By successive diluting method three acidicbacillus strains were isolated from the samples of acid mine drainage taken from Dexing Copper Mine in Jiangxi province, Daye Copper Mine in Hubei province and Dachang Copper Mine in Guangxi province. The three Acidithiobacillus strains are similar to Acidithiobacillus ferrooxidans in shapes and biological characters. 16S rRNA gene sequence analysis further confirms that the three bacteria strains are Acidithiobacillus ferrooxidans. The results show that anions obviously impact the oxidation capacity of ferrous ions of these bacteria with an effect order as: ＞Cl−＞ . And their tolerances to anions are obviously different from each other.
Key words: Acidithiobacillus ferrooxidans; ; Cl−;
