(1. 中南大學(xué) 資源加工與生物工程學(xué)院，長沙 410083；2. 中南大學(xué) 生物冶金教育部重點(diǎn)實(shí)驗(yàn)室，長沙410083)
摘 要: 從湖北大冶銅礦的銅山口硫化礦礦坑水中分離得到了一株嗜酸兼性異養(yǎng)細(xì)菌，暫命名為DY。該菌株為革蘭氏陰性細(xì)菌，短桿狀，菌體大小為(0.4±0.1)μm×(1.2±0.2)μm，最適生長溫度為30 ℃，最適初始生長pH值為3.5，能利用葡萄糖、乳糖、蔗糖和單質(zhì)硫生長，不能利用FeSO4進(jìn)行生長。其系統(tǒng)發(fā)育樹結(jié)果表明，菌株DY與Acidiphilium cryptum(Y18446)位于系統(tǒng)發(fā)育樹的同一分支中，相似度為99.69%。黃銅礦(CuFeS2)搖瓶細(xì)菌浸出實(shí)驗(yàn)顯示，DY菌株單獨(dú)浸出黃銅礦的能力較弱，但和嗜酸自養(yǎng)的氧化亞鐵硫桿菌ATCC 23270混合浸礦時(shí)，與氧化亞鐵硫桿菌單獨(dú)浸礦相比，30 d后黃銅礦的浸出率提高了35.98%。
關(guān)鍵字: 酸性礦坑廢水；Acidiphilium；黃銅礦；生物浸出
（1. School of Minerals Processing and Bioengineering, Changsha 410083, China;2. Key Laboratory of Biometallurgy of Ministry of Education, Central South University, Changsha 410083, China）
Abstract:An Acidiphilium strain DY was isolated from acid mine drainage of Daye mine, Hubei Province, China. The cell of DY strain is Gram negative and rod-shaped in (0.4±0.1)μm×(1.2±0.2)μm. The optimum growth temperature is 30 ℃, and the optimum growth pH is 3.5. It can grow facultative heterotrophically by using glucose, lactose, sucrose and elemental sulfur as sole energy sources, but can not grow with FeSO4. A phylogenetic tree was constructed with the published 16SrRNA gene sequences of the relative bacteria species. In the phylogenetic tree, the DY strain has the closest relative to the Acidiphilium cryptum (Y18446) with 99.69% sequence similarity. The bioleaching experiment of chalcopyrite revealed that the DY strain has little efficiency of leaching chalcopyrite, but when it is coupled with acidophilic autotrophilic bacteria Acidithiobacillus ferrooxidans ATCC 23270, it can increase the chalcopyrite leaching efficiency by 35.98% after 30 d, compared with Acidithiobacillus ferrooxidans ATCC 23270 leaching efficiency.
Key words: acid mine drainage; Acidiphilium; chalcopyrite; bioleaching
